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KMID : 0380420190430010019
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Journal of Prventive Veterinary Medicine
2019 Volume.43 No. 1 p.19 ~ p.25
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Comparison of protective efficacy of Stx2eA toxoid with Salmonella ghosts carrying F18+STEC antigenic proteins against lethal challenge in the murine model
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Won Ga-Yeon
Choi Min-Soo
Lee John-Hwa
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Abstract
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Shiga toxin 2e (Stx2e) has a pivotal role in the colonization and enterotoxicity of F18+Shiga toxin-producing Escherichia coli (STEC), which causes porcine edema disease (ED). In this study, a Stx2eA mutant, which has a Glu167Gln mutation in Stx2eA that inactivates N-glycosidase activity, was genetically engineered to evaluate its potential immunogenicity and protective efficacy. A significant increase in serum IgG1 (a Th2 indicator) was shown in mice immunized with the mutated Stx2eA. However, only 56% of the mice immunized with the toxoid (5 μg) survived following a challenge with a lethal dose 50 (LD50) of a virulent F18+STEC strain (JOL654), while mice immunized with Salmonella ghosts delivering selected antigens of F18+STEC showed an 86% survival rate. The results suggest that sole use of the mutated Stx2eA toxoid may not be an effective preventive strategy for the control of porcine ED.
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KEYWORD
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Stx2eA, porcine edema disease, toxoid, F18+STEC, Salmonella ghost
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